gelatin veronal buffered saline gvb Search Results


cacl 2 gvb  (Boston BioProducts)
94
Boston BioProducts cacl 2 gvb
Cacl 2 Gvb, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gvbe  (Thermo Fisher)
99
Thermo Fisher gvbe
Gvbe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mgcl2  (Boston BioProducts)
86
Boston BioProducts mgcl2
Mgcl2, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gelatin veronal-buffered saline (gvbs)  (CompTech Computer Technologies)
90
CompTech Computer Technologies gelatin veronal-buffered saline (gvbs)
Gelatin Veronal Buffered Saline (Gvbs), supplied by CompTech Computer Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mg-egta-gvbs  (CompTech Computer Technologies)
90
CompTech Computer Technologies mg-egta-gvbs
Functional hemolytic assays of complement activation. (A) CH50-type antibody-initiated complement assays incubating antibody-sensitized erythrocytes (EA) with 0.2% human serum for 1 hour at 31-41°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There is 2-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01) (P ≤ 0.05 for the comparisons 31 vs. 37, 31 vs. 39, 31 vs. 41, 33 vs. 37, 33 vs. 39, 33 vs. 41, 35 vs. 39, 35 vs. 41, with significantly more hemolysis at lower temperatures) Data are mean ± SEM for 4 independent experiments. (B) Evaluating antibody-initiated complement activation from 0-37°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There was 2.5-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01). (P ≤ 0.05 for the comparisons 31 vs. 37, 24 vs. 37, 19 vs. 37, with significantly more hemolysis at lower temperatures). The trend of increased complement activation was reversed at 13°C. (No statistical difference between hemolysis at 13 vs. 37 or 7 vs. 37). Data are mean ± SEM for 4 independent experiments. (C) AP50-type alternative complement pathway assays, incubating rabbit erythrocytes with 4% human serum in <t>Mg-EGTA-GVBS</t> for 30 minutes at 31-41°C showed that temperature did not influence complement-mediated cell lysis over therapeutic hypothermia temperatures (ANOVA P = 0.45). Data are mean ± SEM for 4 independent experiments. (D) Evaluating alternative pathway activation from 0-37°C showed that alternative pathway activation was significantly inhibited at 24°C and below. There was no difference in the degree of hemolysis at 31°C vs. 37°C) Data are mean ± SEM for 4 independent experiments.
Mg Egta Gvbs, supplied by CompTech Computer Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gvbs ++ buffer  (Lonza)
90
Lonza gvbs ++ buffer
Functional hemolytic assays of complement activation. (A) CH50-type antibody-initiated complement assays incubating antibody-sensitized erythrocytes (EA) with 0.2% human serum for 1 hour at 31-41°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There is 2-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01) (P ≤ 0.05 for the comparisons 31 vs. 37, 31 vs. 39, 31 vs. 41, 33 vs. 37, 33 vs. 39, 33 vs. 41, 35 vs. 39, 35 vs. 41, with significantly more hemolysis at lower temperatures) Data are mean ± SEM for 4 independent experiments. (B) Evaluating antibody-initiated complement activation from 0-37°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There was 2.5-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01). (P ≤ 0.05 for the comparisons 31 vs. 37, 24 vs. 37, 19 vs. 37, with significantly more hemolysis at lower temperatures). The trend of increased complement activation was reversed at 13°C. (No statistical difference between hemolysis at 13 vs. 37 or 7 vs. 37). Data are mean ± SEM for 4 independent experiments. (C) AP50-type alternative complement pathway assays, incubating rabbit erythrocytes with 4% human serum in <t>Mg-EGTA-GVBS</t> for 30 minutes at 31-41°C showed that temperature did not influence complement-mediated cell lysis over therapeutic hypothermia temperatures (ANOVA P = 0.45). Data are mean ± SEM for 4 independent experiments. (D) Evaluating alternative pathway activation from 0-37°C showed that alternative pathway activation was significantly inhibited at 24°C and below. There was no difference in the degree of hemolysis at 31°C vs. 37°C) Data are mean ± SEM for 4 independent experiments.
Gvbs ++ Buffer, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gvb (gelatin veronal buffer  (Millipore)
90
Millipore gvb (gelatin veronal buffer
Functional hemolytic assays of complement activation. (A) CH50-type antibody-initiated complement assays incubating antibody-sensitized erythrocytes (EA) with 0.2% human serum for 1 hour at 31-41°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There is 2-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01) (P ≤ 0.05 for the comparisons 31 vs. 37, 31 vs. 39, 31 vs. 41, 33 vs. 37, 33 vs. 39, 33 vs. 41, 35 vs. 39, 35 vs. 41, with significantly more hemolysis at lower temperatures) Data are mean ± SEM for 4 independent experiments. (B) Evaluating antibody-initiated complement activation from 0-37°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There was 2.5-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01). (P ≤ 0.05 for the comparisons 31 vs. 37, 24 vs. 37, 19 vs. 37, with significantly more hemolysis at lower temperatures). The trend of increased complement activation was reversed at 13°C. (No statistical difference between hemolysis at 13 vs. 37 or 7 vs. 37). Data are mean ± SEM for 4 independent experiments. (C) AP50-type alternative complement pathway assays, incubating rabbit erythrocytes with 4% human serum in <t>Mg-EGTA-GVBS</t> for 30 minutes at 31-41°C showed that temperature did not influence complement-mediated cell lysis over therapeutic hypothermia temperatures (ANOVA P = 0.45). Data are mean ± SEM for 4 independent experiments. (D) Evaluating alternative pathway activation from 0-37°C showed that alternative pathway activation was significantly inhibited at 24°C and below. There was no difference in the degree of hemolysis at 31°C vs. 37°C) Data are mean ± SEM for 4 independent experiments.
Gvb (Gelatin Veronal Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gelatin veronal buffered saline  (Valiant Co Ltd)
94
Valiant Co Ltd gelatin veronal buffered saline
Functional hemolytic assays of complement activation. (A) CH50-type antibody-initiated complement assays incubating antibody-sensitized erythrocytes (EA) with 0.2% human serum for 1 hour at 31-41°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There is 2-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01) (P ≤ 0.05 for the comparisons 31 vs. 37, 31 vs. 39, 31 vs. 41, 33 vs. 37, 33 vs. 39, 33 vs. 41, 35 vs. 39, 35 vs. 41, with significantly more hemolysis at lower temperatures) Data are mean ± SEM for 4 independent experiments. (B) Evaluating antibody-initiated complement activation from 0-37°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There was 2.5-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01). (P ≤ 0.05 for the comparisons 31 vs. 37, 24 vs. 37, 19 vs. 37, with significantly more hemolysis at lower temperatures). The trend of increased complement activation was reversed at 13°C. (No statistical difference between hemolysis at 13 vs. 37 or 7 vs. 37). Data are mean ± SEM for 4 independent experiments. (C) AP50-type alternative complement pathway assays, incubating rabbit erythrocytes with 4% human serum in <t>Mg-EGTA-GVBS</t> for 30 minutes at 31-41°C showed that temperature did not influence complement-mediated cell lysis over therapeutic hypothermia temperatures (ANOVA P = 0.45). Data are mean ± SEM for 4 independent experiments. (D) Evaluating alternative pathway activation from 0-37°C showed that alternative pathway activation was significantly inhibited at 24°C and below. There was no difference in the degree of hemolysis at 31°C vs. 37°C) Data are mean ± SEM for 4 independent experiments.
Gelatin Veronal Buffered Saline, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gelatin veronal buffered saline - by Bioz Stars, 2026-03
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gelatin veronal buffered saline  (Boston BioProducts)
96
Boston BioProducts gelatin veronal buffered saline
Functional hemolytic assays of complement activation. (A) CH50-type antibody-initiated complement assays incubating antibody-sensitized erythrocytes (EA) with 0.2% human serum for 1 hour at 31-41°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There is 2-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01) (P ≤ 0.05 for the comparisons 31 vs. 37, 31 vs. 39, 31 vs. 41, 33 vs. 37, 33 vs. 39, 33 vs. 41, 35 vs. 39, 35 vs. 41, with significantly more hemolysis at lower temperatures) Data are mean ± SEM for 4 independent experiments. (B) Evaluating antibody-initiated complement activation from 0-37°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There was 2.5-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01). (P ≤ 0.05 for the comparisons 31 vs. 37, 24 vs. 37, 19 vs. 37, with significantly more hemolysis at lower temperatures). The trend of increased complement activation was reversed at 13°C. (No statistical difference between hemolysis at 13 vs. 37 or 7 vs. 37). Data are mean ± SEM for 4 independent experiments. (C) AP50-type alternative complement pathway assays, incubating rabbit erythrocytes with 4% human serum in <t>Mg-EGTA-GVBS</t> for 30 minutes at 31-41°C showed that temperature did not influence complement-mediated cell lysis over therapeutic hypothermia temperatures (ANOVA P = 0.45). Data are mean ± SEM for 4 independent experiments. (D) Evaluating alternative pathway activation from 0-37°C showed that alternative pathway activation was significantly inhibited at 24°C and below. There was no difference in the degree of hemolysis at 31°C vs. 37°C) Data are mean ± SEM for 4 independent experiments.
Gelatin Veronal Buffered Saline, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
gelatin veronal buffered saline - by Bioz Stars, 2026-03
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gvbs  (Millipore)
90
Millipore gvbs
Functional hemolytic assays of complement activation. (A) CH50-type antibody-initiated complement assays incubating antibody-sensitized erythrocytes (EA) with 0.2% human serum for 1 hour at 31-41°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There is 2-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01) (P ≤ 0.05 for the comparisons 31 vs. 37, 31 vs. 39, 31 vs. 41, 33 vs. 37, 33 vs. 39, 33 vs. 41, 35 vs. 39, 35 vs. 41, with significantly more hemolysis at lower temperatures) Data are mean ± SEM for 4 independent experiments. (B) Evaluating antibody-initiated complement activation from 0-37°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There was 2.5-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01). (P ≤ 0.05 for the comparisons 31 vs. 37, 24 vs. 37, 19 vs. 37, with significantly more hemolysis at lower temperatures). The trend of increased complement activation was reversed at 13°C. (No statistical difference between hemolysis at 13 vs. 37 or 7 vs. 37). Data are mean ± SEM for 4 independent experiments. (C) AP50-type alternative complement pathway assays, incubating rabbit erythrocytes with 4% human serum in <t>Mg-EGTA-GVBS</t> for 30 minutes at 31-41°C showed that temperature did not influence complement-mediated cell lysis over therapeutic hypothermia temperatures (ANOVA P = 0.45). Data are mean ± SEM for 4 independent experiments. (D) Evaluating alternative pathway activation from 0-37°C showed that alternative pathway activation was significantly inhibited at 24°C and below. There was no difference in the degree of hemolysis at 31°C vs. 37°C) Data are mean ± SEM for 4 independent experiments.
Gvbs, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gelatin  (Bio-Rad)
96
Bio-Rad gelatin
Functional hemolytic assays of complement activation. (A) CH50-type antibody-initiated complement assays incubating antibody-sensitized erythrocytes (EA) with 0.2% human serum for 1 hour at 31-41°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There is 2-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01) (P ≤ 0.05 for the comparisons 31 vs. 37, 31 vs. 39, 31 vs. 41, 33 vs. 37, 33 vs. 39, 33 vs. 41, 35 vs. 39, 35 vs. 41, with significantly more hemolysis at lower temperatures) Data are mean ± SEM for 4 independent experiments. (B) Evaluating antibody-initiated complement activation from 0-37°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There was 2.5-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01). (P ≤ 0.05 for the comparisons 31 vs. 37, 24 vs. 37, 19 vs. 37, with significantly more hemolysis at lower temperatures). The trend of increased complement activation was reversed at 13°C. (No statistical difference between hemolysis at 13 vs. 37 or 7 vs. 37). Data are mean ± SEM for 4 independent experiments. (C) AP50-type alternative complement pathway assays, incubating rabbit erythrocytes with 4% human serum in <t>Mg-EGTA-GVBS</t> for 30 minutes at 31-41°C showed that temperature did not influence complement-mediated cell lysis over therapeutic hypothermia temperatures (ANOVA P = 0.45). Data are mean ± SEM for 4 independent experiments. (D) Evaluating alternative pathway activation from 0-37°C showed that alternative pathway activation was significantly inhibited at 24°C and below. There was no difference in the degree of hemolysis at 31°C vs. 37°C) Data are mean ± SEM for 4 independent experiments.
Gelatin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gelatin veronal buffer  (Millipore)
90
Millipore gelatin veronal buffer
Functional hemolytic assays of complement activation. (A) CH50-type antibody-initiated complement assays incubating antibody-sensitized erythrocytes (EA) with 0.2% human serum for 1 hour at 31-41°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There is 2-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01) (P ≤ 0.05 for the comparisons 31 vs. 37, 31 vs. 39, 31 vs. 41, 33 vs. 37, 33 vs. 39, 33 vs. 41, 35 vs. 39, 35 vs. 41, with significantly more hemolysis at lower temperatures) Data are mean ± SEM for 4 independent experiments. (B) Evaluating antibody-initiated complement activation from 0-37°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There was 2.5-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01). (P ≤ 0.05 for the comparisons 31 vs. 37, 24 vs. 37, 19 vs. 37, with significantly more hemolysis at lower temperatures). The trend of increased complement activation was reversed at 13°C. (No statistical difference between hemolysis at 13 vs. 37 or 7 vs. 37). Data are mean ± SEM for 4 independent experiments. (C) AP50-type alternative complement pathway assays, incubating rabbit erythrocytes with 4% human serum in <t>Mg-EGTA-GVBS</t> for 30 minutes at 31-41°C showed that temperature did not influence complement-mediated cell lysis over therapeutic hypothermia temperatures (ANOVA P = 0.45). Data are mean ± SEM for 4 independent experiments. (D) Evaluating alternative pathway activation from 0-37°C showed that alternative pathway activation was significantly inhibited at 24°C and below. There was no difference in the degree of hemolysis at 31°C vs. 37°C) Data are mean ± SEM for 4 independent experiments.
Gelatin Veronal Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gelatin veronal buffer/product/Millipore
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Image Search Results


Functional hemolytic assays of complement activation. (A) CH50-type antibody-initiated complement assays incubating antibody-sensitized erythrocytes (EA) with 0.2% human serum for 1 hour at 31-41°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There is 2-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01) (P ≤ 0.05 for the comparisons 31 vs. 37, 31 vs. 39, 31 vs. 41, 33 vs. 37, 33 vs. 39, 33 vs. 41, 35 vs. 39, 35 vs. 41, with significantly more hemolysis at lower temperatures) Data are mean ± SEM for 4 independent experiments. (B) Evaluating antibody-initiated complement activation from 0-37°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There was 2.5-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01). (P ≤ 0.05 for the comparisons 31 vs. 37, 24 vs. 37, 19 vs. 37, with significantly more hemolysis at lower temperatures). The trend of increased complement activation was reversed at 13°C. (No statistical difference between hemolysis at 13 vs. 37 or 7 vs. 37). Data are mean ± SEM for 4 independent experiments. (C) AP50-type alternative complement pathway assays, incubating rabbit erythrocytes with 4% human serum in Mg-EGTA-GVBS for 30 minutes at 31-41°C showed that temperature did not influence complement-mediated cell lysis over therapeutic hypothermia temperatures (ANOVA P = 0.45). Data are mean ± SEM for 4 independent experiments. (D) Evaluating alternative pathway activation from 0-37°C showed that alternative pathway activation was significantly inhibited at 24°C and below. There was no difference in the degree of hemolysis at 31°C vs. 37°C) Data are mean ± SEM for 4 independent experiments.

Journal: Journal of Translational Medicine

Article Title: Clinical hypothermia temperatures increase complement activation and cell destruction via the classical pathway

doi: 10.1186/1479-5876-12-181

Figure Lengend Snippet: Functional hemolytic assays of complement activation. (A) CH50-type antibody-initiated complement assays incubating antibody-sensitized erythrocytes (EA) with 0.2% human serum for 1 hour at 31-41°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There is 2-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01) (P ≤ 0.05 for the comparisons 31 vs. 37, 31 vs. 39, 31 vs. 41, 33 vs. 37, 33 vs. 39, 33 vs. 41, 35 vs. 39, 35 vs. 41, with significantly more hemolysis at lower temperatures) Data are mean ± SEM for 4 independent experiments. (B) Evaluating antibody-initiated complement activation from 0-37°C showed that hemolysis of antibody-sensitized erythrocytes in human serum differed significantly as a function of temperature (ANOVA P < 0.01). There was 2.5-fold more hemolysis at 31°C vs. 37°C (* t -test P = 0.01). (P ≤ 0.05 for the comparisons 31 vs. 37, 24 vs. 37, 19 vs. 37, with significantly more hemolysis at lower temperatures). The trend of increased complement activation was reversed at 13°C. (No statistical difference between hemolysis at 13 vs. 37 or 7 vs. 37). Data are mean ± SEM for 4 independent experiments. (C) AP50-type alternative complement pathway assays, incubating rabbit erythrocytes with 4% human serum in Mg-EGTA-GVBS for 30 minutes at 31-41°C showed that temperature did not influence complement-mediated cell lysis over therapeutic hypothermia temperatures (ANOVA P = 0.45). Data are mean ± SEM for 4 independent experiments. (D) Evaluating alternative pathway activation from 0-37°C showed that alternative pathway activation was significantly inhibited at 24°C and below. There was no difference in the degree of hemolysis at 31°C vs. 37°C) Data are mean ± SEM for 4 independent experiments.

Article Snippet: Alternative complement pathway hemolytic assays were performed by incubating rabbit erythrocytes (CompTech, Tyler, TX) with 4% NHS in Mg-EGTA-GVBS at temperatures ranging from 0°C to 37°C.

Techniques: Functional Assay, Activation Assay, Lysis